Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase
Identifieur interne : 004311 ( Main/Exploration ); précédent : 004310; suivant : 004312Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase
Auteurs : Magali Frugier [France] ; Catherine Florentz [France] ; Mir Wais Hosseini [France] ; Jean-Marie Lehn [France] ; Richard Giegé [France]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1994.
Descripteurs français
- KwdFr :
- ARN de transfert de la valine (), ARN de transfert de la valine (biosynthèse), Bactériophage T7 (enzymologie), Cinétique, Conformation d'acide nucléique, DNA-directed RNA polymerases (), DNA-directed RNA polymerases (métabolisme), Données de séquences moléculaires, Matrices (génétique), Oligodésoxyribonucléotides, Polyamines (), Polyamines (pharmacologie), Protéines virales, Relation structure-activité, Régions promotrices (génétique), Structure moléculaire, Séquence nucléotidique, Transcription génétique ().
- MESH :
- biosynthèse : ARN de transfert de la valine.
- enzymologie : Bactériophage T7.
- métabolisme : DNA-directed RNA polymerases.
- pharmacologie : Polyamines.
- ARN de transfert de la valine, Cinétique, Conformation d'acide nucléique, DNA-directed RNA polymerases, Données de séquences moléculaires, Matrices (génétique), Oligodésoxyribonucléotides, Polyamines, Protéines virales, Relation structure-activité, Régions promotrices (génétique), Structure moléculaire, Séquence nucléotidique, Transcription génétique.
- Pascal (Inist)
English descriptors
- KwdEn :
- Activity concentration relation, Bacteriophage T7 (enzymology), Base Sequence, DNA-Directed RNA Polymerases (drug effects), DNA-Directed RNA Polymerases (metabolism), DNA-directed RNA polymerase, In vitro transcription, Kinetics, Mechanism of action, Molecular Sequence Data, Molecular Structure, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Phage T7, Polyamine, Polyamines (chemistry), Polyamines (pharmacology), Promoter Regions, Genetic, RNA, Transfer, Val (biosynthesis), RNA, Transfer, Val (chemistry), Structure activity relation, Structure-Activity Relationship, Synthetic product, Templates, Genetic, Transcription, Genetic (drug effects), Viral Proteins.
- MESH :
- chemical , biosynthesis : RNA, Transfer, Val.
- chemical , chemistry : Polyamines, RNA, Transfer, Val.
- chemical , drug effects : DNA-Directed RNA Polymerases.
- drug effects : Transcription, Genetic.
- enzymology : Bacteriophage T7.
- chemical , metabolism : DNA-Directed RNA Polymerases.
- chemical , pharmacology : Polyamines.
- Base Sequence, Kinetics, Molecular Sequence Data, Molecular Structure, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Structure-Activity Relationship, Templates, Genetic, Viral Proteins.
Abstract
The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts 25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38JN6C10.
Url:
DOI: 10.1093/nar/22.14.2784
Affiliations:
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Le document en format XML
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type="ISSN">0305-1048</idno><imprint><publisher>Oxford University Press</publisher><date type="published">1994</date><biblScope unit="vol">22</biblScope><biblScope unit="issue">14</biblScope><biblScope unit="page" from="2784">2784</biblScope><biblScope unit="page" to="2790">2790</biblScope></imprint><idno type="ISSN">0305-1048</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activity concentration relation</term><term>Bacteriophage T7 (enzymology)</term><term>Base Sequence</term><term>DNA-Directed RNA Polymerases (drug effects)</term><term>DNA-Directed RNA Polymerases (metabolism)</term><term>DNA-directed RNA polymerase</term><term>In vitro transcription</term><term>Kinetics</term><term>Mechanism of action</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Nucleic Acid Conformation</term><term>Oligodeoxyribonucleotides</term><term>Phage T7</term><term>Polyamine</term><term>Polyamines (chemistry)</term><term>Polyamines (pharmacology)</term><term>Promoter Regions, Genetic</term><term>RNA, Transfer, Val (biosynthesis)</term><term>RNA, Transfer, Val (chemistry)</term><term>Structure activity relation</term><term>Structure-Activity Relationship</term><term>Synthetic product</term><term>Templates, Genetic</term><term>Transcription, Genetic (drug effects)</term><term>Viral Proteins</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN de transfert de la valine ()</term><term>ARN de transfert de la valine (biosynthèse)</term><term>Bactériophage T7 (enzymologie)</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>DNA-directed RNA polymerases ()</term><term>DNA-directed RNA polymerases (métabolisme)</term><term>Données de séquences moléculaires</term><term>Matrices (génétique)</term><term>Oligodésoxyribonucléotides</term><term>Polyamines ()</term><term>Polyamines (pharmacologie)</term><term>Protéines virales</term><term>Relation structure-activité</term><term>Régions promotrices (génétique)</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term><term>Transcription génétique ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Transfer, Val</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Polyamines</term><term>RNA, Transfer, Val</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>DNA-Directed RNA Polymerases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN de transfert de la valine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bactériophage T7</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacteriophage T7</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Directed RNA Polymerases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DNA-directed RNA polymerases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Polyamines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Polyamines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Nucleic Acid Conformation</term><term>Oligodeoxyribonucleotides</term><term>Promoter Regions, Genetic</term><term>Structure-Activity Relationship</term><term>Templates, Genetic</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN de transfert de la valine</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>DNA-directed RNA polymerases</term><term>Données de séquences moléculaires</term><term>Matrices (génétique)</term><term>Oligodésoxyribonucléotides</term><term>Polyamines</term><term>Protéines virales</term><term>Relation structure-activité</term><term>Régions promotrices (génétique)</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term><term>Transcription génétique</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Bactériophage T7</term><term>DNA-directed RNA polymerase</term><term>Mécanisme action</term><term>Polyamine</term><term>Produit synthétique</term><term>Relation concentration activité</term><term>Relation structure activité</term><term>Transcription in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts 25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38JN6C10.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Strasbourg</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Frugier, Magali" sort="Frugier, Magali" uniqKey="Frugier M" first="Magali" last="Frugier">Magali Frugier</name></region><name sortKey="Florentz, Catherine" sort="Florentz, Catherine" uniqKey="Florentz C" first="Catherine" last="Florentz">Catherine Florentz</name><name sortKey="Giege, Richard" sort="Giege, Richard" uniqKey="Giege R" first="Richard" last="Giegé">Richard Giegé</name><name sortKey="Hosseini, Mir Wais" sort="Hosseini, Mir Wais" uniqKey="Hosseini M" first="Mir Wais" last="Hosseini">Mir Wais Hosseini</name><name sortKey="Lehn, Jean Marie" sort="Lehn, Jean Marie" uniqKey="Lehn J" first="Jean-Marie" last="Lehn">Jean-Marie Lehn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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